More Resolution, Less Wait With Biomolex 700

Biomolex's unique technology offers new tools and enables new methods in autoradiography for researchers in the field of proteomics and tissue imaging. The technology supports detection of biological samples labeled with radionuclides. Compared to traditional detection systems for autoradiograpy like film and phosphorus screens, Biomolex technology offers advantages such as real-time detection through single event counting, energy registration, signal linearity over 5 logs, increased sensitivity, improved image resolution, dual isotope imaging capabilities and a small footprint.

Radionuclides Imaging and Quantification

The Biomolex Microarray Reader is the first commercial product using a silicon strip detector for biomedical imaging and the first instrument ever enabling real-time detection and subsequent separation based on registered energy spectra from different β-emitters. Signals from different β-emitters for any chosen region on the sensor can be separated and ratios of the different isotopes can be calculated with advanced statistical algorithms.

Kinase Microarrays

The Biomolex 700 has been used with PepChip arrays (Pepscan Presto BV) to identify kinase profiles in the breast cancer cell line BT - 474. The analyses have identified several kinases that may play an important roll in the observed drug resistance to EGFR-inhibitor Iressa (work done in collaboration with Eivind Hovig at The Norwegian Radium Hospital).

Tissue Autoradiography

Audoradiography is a recognized method for imaging distributions of radiolabelled pharmaceuticals or bio molecules in biological tissues. Traditional methods like film and phosphor plates suffer from poor quantification and lack real-time read-out and energy registration. The new technology developed by Biomolex enables detailed imaging with high sensitivity and high spatial resolution which are both crucial to get accurate knowledge of transport and the uptake of labeled biomolecules. With a resolution of <50μm, the Biomolex 700 allows the transport and the uptake to be identified and quantified providing high quality images to verify pharmacokinetic models.